Quantitative analysis of gentiobiose and isomaltose in admixture, and its application to the characterization of dextrins

Gentiobiose in admixture with isomaltose was selectively hydrolyzed by emulsin, and the resultant d-glucose was separated from unchanged isomaltose by thin-layer chromatography on borohydride-reduced microcellulose. The separated sugars were located, eluted, and assayed photometrically by the anthro...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate research 1969-01, Vol.9 (4), p.407-413
Main Authors: Wolfrom, M.L., Schwab, G.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Gentiobiose in admixture with isomaltose was selectively hydrolyzed by emulsin, and the resultant d-glucose was separated from unchanged isomaltose by thin-layer chromatography on borohydride-reduced microcellulose. The separated sugars were located, eluted, and assayed photometrically by the anthrone method. Representative, acid-modified dextrins were partially acid-hydrolyzed under conditions wherein reversion was negligible, and disaccharide formation was maximal. Inorganic salt was removed by extraction of the neutralized, evaporated hydrolyzate with N,N-dimethylformamide under anhydrous conditions, and the extract was analyzed by the above method without solvent removal. The method gives comparable results for the characterization of dextrination and is more rapid than those previously utilized.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(00)80025-7