3-Dimensional super-resolution by spectrally selective imaging

The mutual spatial positions of individual pentacene molecules embedded in a p-terphenyl host crystal, residing within the same diffraction-limited volume, are determined with far-field optics with an accuracy far below the Rayleigh distance. This is achieved by spectrally selective imaging, an appr...

Full description

Saved in:
Bibliographic Details
Published in:Chemical physics letters 1998-07, Vol.292 (1), p.183-187
Main Authors: van Oijen, A.M, Köhler, J, Schmidt, J, Müller, M, Brakenhoff, G.J
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mutual spatial positions of individual pentacene molecules embedded in a p-terphenyl host crystal, residing within the same diffraction-limited volume, are determined with far-field optics with an accuracy far below the Rayleigh distance. This is achieved by spectrally selective imaging, an approach based on the combination of confocal microscopy, single-molecule detection and position-sensitive imaging. It is demonstrated that the molecules can be localized in three dimensions with a precision of 40 nm in the lateral and 100 nm in the axial dimension which represents an enhancement by a factor of 20 and 65, respectively, compared to the Rayleigh distance at the used numerical aperture of the microscope.
ISSN:0009-2614
1873-4448
DOI:10.1016/S0009-2614(98)00673-3