Responses of thiols to an oxidant challenge: differences between blood and tissues in the rat

• Published in: Chemico-biological interactions 2001-03, Vol.134 (1), p.73-85
• Main Authors: Giannerini, Fabiola, Giustarini, Daniela, Lusini, Lorenzo, Rossi, Ranieri, Di Simplicio, Paolo
• Format: Article
• Language: English
• Subjects: Animals; Blood Proteins - drug effects; Blood Proteins - metabolism; Diamide; Diamide - pharmacology; Disulfides - metabolism; Glutathione - metabolism; Glutathione Disulfide - metabolism; GSH; Kidney - metabolism; Liver - metabolism; Lung - metabolism; Male; Malondialdehyde - metabolism; Myocardium - metabolism; Organ Specificity - drug effects; Oxidants - pharmacology; Oxidative stress; Protein S-thiolation; Rat organs; Rats; Rats, Wistar; Spleen - metabolism; Sulfhydryl Compounds - analysis; Sulfhydryl Compounds - metabolism; Sulfhydryl Reagents - metabolism; Sulfhydryl Reagents - pharmacology; tert-Butylhydroperoxide - pharmacology; Testis - metabolism
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/S0009-2797(00)00316-1

Treatment of rats with diamide (100 mg/kg i.p.) altered the thiol components of the blood to a very different extent than in tissues (liver, kidney, lung, spleen, heart and testis). A total consumption (10 min) and regeneration (120 min) of blood glutathione (GSH), matched by a parallel increase and decrease in glutathione-protein mixed disulfides (GS-SP) was observed. In contrast, no modification of non-protein SH groups (NPSH) and protein SH groups (PSH), GS-SP and malondialdehyde (MDA) was observed in liver, kidney, lung, testis spleen and heart within same time range. In particular, only glutathione disulfide (GSSG) levels and some activities of antioxidant enzymes were modified to a small extent and in an opposite direction in some organs. For example, GSSG, and glucose-6-phosphate dehydrogenase (G-6-PDH) and catalase (CAT) activities appeared up-regulated in one tissue and down-regulated in another. The least modified organ was the liver, whereas lung and spleen were the most affected (lung, GSSG, significantly increased whereas G-6-PDH, glutaredoxin (GRX), GPX, superoxide dimutase (SOD) levels were significantly lowered; spleen, GSSG and the activity of glutathione reductase (GR), G-6-PDH and glutathione transferase (GST) were significantly decreased). The different responses of erythrocytes and organs to diamide were explained by the high affinity of hemoglobin and by the relatively high potential of thiol regeneration in organs. The rapid reversibility of the process of protein S-thiolation in blood and the small effects in organs leads us to propose the existence of an inter-organ cooperation in the rat that regulates protein S-thiolation in blood. Plasma thiols may well play a role in this process.