The toxicity of opiates and their metabolites in HepG2 cells

The toxicity of codeine (C), codeinone (CO), morphine (M), oxycodone (OC), pholcodine (P) and pholcodine-N-oxide (P-NOX) was assessed in HepG2 cells by determining cell viability via the measurement of lactate dehydrogenase (LDH) leakage through the membrane, depletion of reduced glutathione (GSH) a...

Full description

Saved in:
Bibliographic Details
Published in:Chemico-biological interactions 2003-10, Vol.146 (2), p.121-129
Main Authors: Jairaj, Mark, Watson, David G., Grant, M.Helen, Skellern, Graham G.
Format: Article
Language:English
Subjects:
Carcinoma, Hepatocellular
Cell Line, Tumor
Cell Survival - drug effects
Chromatography, Liquid
Codeine
Codeinone–glutathione conjugate
Glutathione - metabolism
Hepatocytes - drug effects
Hepatocytes - metabolism
Hepatocytes - pathology
HepG2 cell
Humans
L-Lactate Dehydrogenase - metabolism
Liver Neoplasms
Narcotics - chemistry
Narcotics - metabolism
Narcotics - toxicity
Proteins - metabolism
Spectrometry, Mass, Electrospray Ionization
Structure-Activity Relationship
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The toxicity of codeine (C), codeinone (CO), morphine (M), oxycodone (OC), pholcodine (P) and pholcodine-N-oxide (P-NOX) was assessed in HepG2 cells by determining cell viability via the measurement of lactate dehydrogenase (LDH) leakage through the membrane, depletion of reduced glutathione (GSH) and measurement of total protein content. Incubation of C, M, OC, P or P-NOX with HepG2 cells resulted in no significant loss of cell viability, depletion of GSH or decreased total protein content. In contrast, with CO there was a marked depletion of GSH with significant differences from control cells (P
ISSN:0009-2797
1872-7786
DOI:10.1016/S0009-2797(03)00091-7