Effect of methylglyoxal on Bufo bufo embryo development: morphological and biochemical aspects

Methylglyoxal (2-oxopropanal) is a cytotoxic compound that can be formed endogenously as a by-product of glycolytic pathway; so its concentration is expected to increase when glycolysis activity increases such as during embryo development. In this work we study the effect of exogenous methylglyoxal...

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Bibliographic Details
Published in:Chemico-biological interactions 1998-07, Vol.114 (3), p.177-189
Main Authors: Amicarelli, F, Bonfigli, A, Colafarina, S, Bucciarelli, T, Principato, G, Ragnelli, A.M, Di Ilio, C, Miranda, M
Format: Article
Language:English
Subjects:
Animals
Bufo bufo
Cell Differentiation - drug effects
Cell division
Cell Division - drug effects
Cytosol - drug effects
Cytosol - metabolism
Embryo development
Embryo, Nonmammalian - drug effects
Embryo, Nonmammalian - enzymology
Embryo, Nonmammalian - metabolism
Embryonic Development
Glutathione - metabolism
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
Glyoxalase system
Indicators and Reagents
Methylglyoxal
Proteins - metabolism
Pyruvaldehyde - pharmacology
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Summary:Methylglyoxal (2-oxopropanal) is a cytotoxic compound that can be formed endogenously as a by-product of glycolytic pathway; so its concentration is expected to increase when glycolysis activity increases such as during embryo development. In this work we study the effect of exogenous methylglyoxal on development and embryo viability during Bufo Bufo development and on the enzymes and cofactors involved in its detoxication process (glyoxalase I and II, reduced glutathione and glyceraldehyde 3-phosphate dehydrogenase). The results show that exogenous methylglyoxal does not affect the enzymatic pattern until stage 20, while it induces a significant activity decrease of the tested enzymes at stage 25. On the contrary methylglyoxal positively influences the reduced glutathione concentration at all the considered stages. At morphological and histological levels methylglyoxal causes a strong retardation of cell division in the early stages, that results in various abnormalities in the late development. In conclusion, methylglyoxal enters the embryo and is antiproliferative and teratogenic: the data further supports the hypothesis of the importance of the glyoxalase system in the process of cell growth and division.
ISSN:0009-2797
1872-7786
DOI:10.1016/S0009-2797(98)00048-9