Simultaneous HPLC Determination of Four Key Metabolites in the Metabolic Pathway for Production of 1,3-Propanediol from Glycerol

• Published in: Chromatographia 2007-05, Vol.65 (9-10), p.629-632
• Main Authors: Chen, Hongwen, Fang, Baishan, Hu, Zongding
• Format: Article
• Language: English
• Subjects: 1,3-Propanediol; 3-Hydroxypropionaldehyde; Analytical chemistry; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Chemistry; Chromatographic methods and physical methods associated with chromatography; Dihydroxyacetone; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; glycerol; liquid chromatography; Methods. Procedures. Technologies; Other chromatographic methods
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1016/S0009-5893(07)82072-8

A high-performance liquid chromatographic (HPLC) method with refractive-index detection has been developed for simultaneous analysis of glycerol, dihydroxyacetone (DHA), 3-hydroxypropionaldehyde (3-HPA), and 1,3-propanediol (1,3-PD), four key substances in the metabolic pathway for production of 1,3-PD from glycerol by microorganism fermentation. The compounds were separated on a 300 mm x 7.8 mm ion-exclusion column with a 65:35 (v/v) mixture of deionized water and acetonitrile, containing 0.0005 M H₂SO₄, as mobile phase. The flow rate was 0.5 mL min-¹. Under these conditions the retention times of 3-HPA, DHA, glycerol, and 1,3-PD were 6.87, 14.63, 16.37, and 18.50 min, respectively. Relative standard deviations and average recoveries were between 0.42 and 0.63% and between 96.7 and 103.1%, respectively; detection limits were between 0.017 and 0.038 g L-¹. The method enabled separation of these compounds.