Chemiluminescence in inflammatory bowel disease patients: a parameter of inflammatory activity

Background: Reactive oxygen species (ROS) are produced in excess in the inflamed mucosa and peripheral blood of patients with inflammatory bowel disease. These species have emerged as a common pathway of tissue injury in a wide variety of inflammatory and other disease processes. The present study w...

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Bibliographic Details
Published in:Clinica chimica acta 2001-08, Vol.310 (2), p.151-156
Main Authors: Oldenburg, Bas, van Kats-Renaud, Henny, Koningsberger, Jacob C., van Berge Henegouwen, Gerard P., van Asbeck, B.Sweder
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Blood Chemical Analysis - methods
Case-Control Studies
Chemiluminescence
Colitis, Ulcerative - blood
Colitis, Ulcerative - metabolism
Crohn Disease - blood
Crohn Disease - metabolism
Digestive system
Female
Humans
Inflammation - blood
Inflammation - metabolism
Inflammatory bowel disease
Investigative techniques, diagnostic techniques (general aspects)
Luminescent Measurements
Luminol
Male
Medical sciences
Middle Aged
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reactive oxygen species
Reactive Oxygen Species - metabolism
Tetradecanoylphorbol Acetate
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Summary:Background: Reactive oxygen species (ROS) are produced in excess in the inflamed mucosa and peripheral blood of patients with inflammatory bowel disease. These species have emerged as a common pathway of tissue injury in a wide variety of inflammatory and other disease processes. The present study was conducted to assess ROS production and to correlate this with parameters of inflammatory activity. Methods: In 25 patients with Crohn's disease (CD), 20 patients with ulcerative colitis (UC) and 65 age- and sex-matched healthy volunteers ROS production was measured using the whole blood luminol enhanced chemiluminescence assay (LECA). Disease activity was assessed using the Crohn's disease activity index and the Ulcerative Colitis Symptoms Score (UCSS) for CD and UC, respectively. Furthermore, the effect of various scavengers, enzymes and enzyme inhibitors on LECA was studied to assess the contribution of different ROS. Results: LECA was significantly higher in CD and UC patients compared with healthy controls (7.1±4.7 and 9.8±6 vs. 5.2±2.8×10 3 counts per minute (cpm), p
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(01)00569-1