The effects of bismuth, iron, zinc and nitrate on free sulfide in batch cultures seeded with fecal flora

Background: Hydrogen sulfide (H 2S) and methanthiol (CH 3SH) have been implicated as bacterially derived toxins which may be damaging to the gut mucosa. The addition of nitrate and metals that bind sulfide could potentially reduce the concentrations of these toxic gases. In this study, the effects o...

Full description

Saved in:
Bibliographic Details
Published in:Clinica chimica acta 2003-09, Vol.335 (1), p.131-135
Main Authors: Mitsui, Takahiro, Edmond, Laurie M., Magee, Elizabeth A., Cummings, John H.
Format: Article
Language:English
Subjects:
Adult
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Biological and medical sciences
Bismuth - pharmacology
Diet
Feces - chemistry
Feces - microbiology
Human bacterial diseases
Humans
Hydrogen Sulfide - metabolism
Infectious diseases
Iron - pharmacology
Large intestine
Male
Medical sciences
Metal
Nitrate
Nitrates - pharmacology
Sulfide
Zinc - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Hydrogen sulfide (H 2S) and methanthiol (CH 3SH) have been implicated as bacterially derived toxins which may be damaging to the gut mucosa. The addition of nitrate and metals that bind sulfide could potentially reduce the concentrations of these toxic gases. In this study, the effects of iron, zinc, bismuth and nitrate on free H 2S concentrations in fecal batch cultures were investigated. Methods: Stool samples were collected from six healthy subjects. Ten percent fecal slurries was made up with phosphate buffer. One milliliter of fecal slurry and 1 ml of metal solution were added to 28 ml anaerobic broth in a 30-ml vial giving final metal concentrations of 0.1, 0.5 and 1.0 mmol/l. For a control, the metal iron solution was replaced by 1 ml of water. After 24 h of incubation at 37 °C, 1 ml of the supernatant from the broth was distilled by microdistillation and sulfide determined by HPLC using amperometric detection. Results: A significant reduction in H 2S ( P
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(03)00288-2