Nω-Nitro- l-arginine methyl ester protects retinal neurons against N-methyl- d-aspartate-induced neurotoxicity in vivo

We investigated whether the inhibition of nitric oxide (NO) synthesis with N ω-nitro- l-arginine methyl ester ( l-NAME), a competitive inhibitor of NO synthase, affects N-methyl- d-aspartate (NMDA)-induced neurotoxicity in the rat retina in vivo. A single intravitreal injection of NMDA damaged the g...

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Bibliographic Details
Published in:European journal of pharmacology 1997-09, Vol.328 (1), p.45-49
Main Authors: Morizane, Chikako, Adachi, Kei, Furutani, Izumi, Fujita, Yasuhiko, Akaike, Akinori, Kashii, Satoshi, Honda, Yoshihito
Format: Article
Language:English
Subjects:
Biological and medical sciences
Medical sciences
Neurotoxicity
Nitric oxide (NO)
NMDA ( N-methyl- d-aspartate)
Nω-Nitro- l-arginine methyl ester ( l-NAME)
Ophthalmology
Retina
Retinopathies
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Summary:We investigated whether the inhibition of nitric oxide (NO) synthesis with N ω-nitro- l-arginine methyl ester ( l-NAME), a competitive inhibitor of NO synthase, affects N-methyl- d-aspartate (NMDA)-induced neurotoxicity in the rat retina in vivo. A single intravitreal injection of NMDA damaged the ganglion cell layer and the inner plexiform layer without affecting the other retinal layers 7 days after injection. Intravitreal injection of (5 R,10 S)-(+)-5-methyl-10,11-dihydro-5 H-dibenzo[ a, d]cyclo-hepten-5,10-imine hydrogen maleate (MK-801) with NMDA significantly reduced NMDA-induced degeneration of the retina. NMDA-induced degeneration was also prevented by intravitreal injection of l-NAME but not of d-NAME. The protective effect of l-NAME was antagonized by l-arginine. These results suggest that NO plays an important role in NMDA-induced excitotoxic degeneration in the retina.
ISSN:0014-2999
1879-0712
DOI:10.1016/S0014-2999(97)83026-9