A brief photochemically induced oxidative insult causes irreversible lens damage and cataract I. Transparency and epithelial cell layer

Short-term photochemical insult of cultured rat lenses caused by the generation of H 2O 2, O 2 .− and OH . was found to lead to rapid irreversible damage to the epithelial cell layer. This irreversible damage was measured by Trypan blue staining, terminal deoxyribonucleotidyl transferase labeling, D...

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Bibliographic Details
Published in:Experimental eye research 1995-05, Vol.60 (5), p.471-481
Main Authors: Spector, Abraham, Wang, Guo-Ming, Wang, Ren-Rong, Li, Wan-Cheng, Kuszak, Jer R.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biological and medical sciences
Cataract - etiology
Cataract - metabolism
Cataract - pathology
cell viability
Epithelium - metabolism
Epithelium - pathology
hydrogen peroxide
Hydrogen Peroxide - metabolism
hydroxyl radical
Hydroxyl Radical - metabolism
Lens diseases
Lens, Crystalline - metabolism
Lens, Crystalline - pathology
Light - adverse effects
Medical sciences
Microscopy, Electron
Necrosis
Ophthalmology
Oxidative Stress
Rats
Rats, Sprague-Dawley
Riboflavin - adverse effects
superoxide
Superoxides - metabolism
Time Factors
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Summary:Short-term photochemical insult of cultured rat lenses caused by the generation of H 2O 2, O 2 .− and OH . was found to lead to rapid irreversible damage to the epithelial cell layer. This irreversible damage was measured by Trypan blue staining, terminal deoxyribonucleotidyl transferase labeling, DNA laddering and morphological analyses. There appears to be an inverse relationship between the period of photochemical insult and the post-insult time required to observe epithelial cell damage. Insulting periods of a few hours require post-insult intervals of days to observe significant cell damage and weeks before complete cortical cataracts are found. Epithelial cell damage precedes the loss of transparency.
ISSN:0014-4835
1096-0007
DOI:10.1016/S0014-4835(05)80062-4