Mechano-chemical effects of Ca 2+ in cross-linked troponin-C films

Changes in troponin C (TnC) conformation upon Ca 2+ binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca 2+-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC s...

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Bibliographic Details
Published in:FEBS letters 2002-07, Vol.524 (1), p.107-110
Main Authors: Bukatina, Anna E, Morozov, Victor N, Gusev, Nikolai B, Sieck, Gary C
Format: Article
Language:English
Subjects:
Calcium
Calcium-binding protein
Conformational change
Cross-linking
Protein film
Troponin C
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Summary:Changes in troponin C (TnC) conformation upon Ca 2+ binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca 2+-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca 2+ from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca 2+ binding to the Ca 2+/Mg 2+ sites of TnC.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)03013-2