Involvement of in vivo induced cheY-4 gene of Vibrio cholerae in motility, early adherence to intestinal epithelial cells and regulation of virulence factors

• Published in: FEBS letters 2002-12, Vol.532 (1), p.221-226
• Main Authors: Banerjee, Rajat, Das, Soumita, Mukhopadhyay, Kanchan, Nag, Sanjay, Chakrabortty, Amit, Chaudhuri, Keya
• Format: Article
• Language: English
• Subjects: Adherence to epithelial cells; Animals; Bacterial Adhesion; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Bacterial Proteins - pharmacology; Base Sequence; Cell Line; Chemotaxis; Cholera Toxin - biosynthesis; Cholera Toxin - genetics; Cloning, Molecular; Epithelial Cells - microbiology; Epithelial Cells - ultrastructure; Fimbriae Proteins - biosynthesis; Fimbriae Proteins - genetics; Gene Components; Gene Duplication; Gene Expression Regulation, Bacterial; Genes, Bacterial; Guinea Pigs; Intestinal Mucosa - microbiology; Kinetics; Locomotion; Molecular Sequence Data; Motility; Mutation; RNA, Bacterial - biosynthesis; Transcription, Genetic; Vibrio cholerae; Vibrio cholerae - genetics; Vibrio cholerae - metabolism; Vibrio cholerae - pathogenicity; Virulence factors; Virulence Factors - biosynthesis; Virulence Factors - genetics
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(02)03678-5

Using a global transcription profile approach cheY-4 of Vibrio cholerae was identified as an in vivo induced gene. In the present study, duplication of the gene in the chromosome resulted in increased motility, increased chemotactic response towards isolated intestinal mucus layer and stronger adhesion to human intestinal epithelial cell line at an early phase of infection compared to wild type and a null mutant strain. In contrast to the cheY-4 null mutant, duplication of cheY-4 gene resulted in increased expression of ctxAB and tcpA, the two major virulence genes of V. cholerae.