Sodium butyrate inhibits angiogenesis of human intestinal microvascular endothelial cells through COX-2 inhibition

We examined the effect of sodium butyrate on in vitro angiogenesis and cyclooxygenase (COX) expression using primary cultures of human intestinal microvascular endothelial cells (HIMEC). Butyrate inhibited VEGF-induced cellular proliferation, transmigration and tube formation of HIMEC. Butyrate also...

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Bibliographic Details
Published in:FEBS letters 2003-11, Vol.554 (1-2), p.88-94
Main Authors: Ogawa, Hitoshi, Rafiee, Parvaneh, Fisher, Pamela J., Johnson, Nathan A., Otterson, Mary F., Binion, David G.
Format: Article
Language:English
Subjects:
Angiogenesis
Angiogenesis Inhibitors - pharmacology
Butyrates - pharmacology
Cell Division - drug effects
Cell Movement - drug effects
COX, cyclooxygenase
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors - pharmacology
Dinoprostone - biosynthesis
Drug Antagonism
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
eNOS, endothelial nitric oxide synthase
Epoprostenol - biosynthesis
FBS, fetal bovine serum
HIMEC, human intestinal microvascular endothelial cell
HRP, horseradish peroxydase
Human intestinal microvascular endothelial cell
Humans
Intestines - blood supply
Isoenzymes - biosynthesis
Isoenzymes - physiology
LPS, lipopolysaccharide
Membrane Proteins
Microcirculation
Neovascularization, Physiologic - drug effects
NSAID, non-steroidal anti-inflammatory drugs
PG, prostaglandin
Prostaglandin I2
Prostaglandin-Endoperoxide Synthases - biosynthesis
Prostaglandin-Endoperoxide Synthases - physiology
SCFA, short-chain fatty acid
Sodium butyrate
TX, thromboxane
Vascular Endothelial Growth Factor A - pharmacology
VEGF, vascular endothelial growth factor
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Summary:We examined the effect of sodium butyrate on in vitro angiogenesis and cyclooxygenase (COX) expression using primary cultures of human intestinal microvascular endothelial cells (HIMEC). Butyrate inhibited VEGF-induced cellular proliferation, transmigration and tube formation of HIMEC. Butyrate also inhibited COX-2 expression as well as prostaglandin (PG)E2 and PGI2 production, and administration of PGI2 analog partially reversed the effect of butyrate on HIMEC angiogenesis. These results indicate that sodium butyrate inhibits HIMEC angiogenesis through down-regulation of COX-2 expression and PG production, and suggest that anti-angiogenic mechanisms may also be involved in the inhibitory effect of sodium butyrate on tumor growth.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)01110-4