Desensitization of the P2X 2 receptor controlled by alternative splicing

P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X 2 receptor splice variant (P2X 2-2) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X 2 gene. P2X 2-2 is as highly expressed as the o...

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Bibliographic Details
Published in:FEBS letters 1997-03, Vol.404 (2), p.294-298
Main Authors: Brändle, U., Spielmanns, P., Osteroth, R., Sim, J., Surprenant, A., Buell, G., Ruppersberg, J.P., Plinkert, P.K., Zenner, H.-P., Glowatzki, E.
Format: Article
Language:English
Subjects:
Alternative splicing
Desensitization
Genomic sequence
P2X receptor
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Summary:P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X 2 receptor splice variant (P2X 2-2) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X 2 gene. P2X 2-2 is as highly expressed as the original P2X 2 sequence in various tissues. ATP-activated currents mediated by heterologous expressed P2X 2 or P2X 2-2 receptors showed significant differences in desensitization time constants and steady-state currents in the continuous presence of ATP. These results imply functional differences between cells differentially expressing these P2X 2 isoforms. © 1997 Federation of European Biochemical Societies.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00128-2