Demonstration of a tandem pair of complement protein modules in GABA B receptor 1a

We have subcloned and expressed the N-terminal portion of the recently sequenced metabotropic GABA receptor, GABA BR1a. This region of the receptor contains a complement protein-like amino acid sequence. The purified 140-residue recombinant protein fragment was soluble and stable. Mass spectrometry...

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Bibliographic Details
Published in:FEBS letters 1998-08, Vol.432 (3), p.103-108
Main Authors: Hawrot, Edward, Xiao, Yuanyuan, Shi, Qing-luo, Norman, David, Kirkitadze, Marina, Barlow, Paul N.
Format: Article
Language:English
Subjects:
Drug target
Epilepsy
GABA B receptor
SCR
Sushi
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Summary:We have subcloned and expressed the N-terminal portion of the recently sequenced metabotropic GABA receptor, GABA BR1a. This region of the receptor contains a complement protein-like amino acid sequence. The purified 140-residue recombinant protein fragment was soluble and stable. Mass spectrometry indicated formation of four disulfide bonds, as expected if two complement protein modules (CPs, also known as SCRs, Sushi domains) are formed. The circular dichroism spectrum was unusual and characteristic of CPs. Differential scanning calorimetry demonstrated a melting point (64°C), and total enthalpy commensurate with two fully folded domains. We thus conclude that the 1a subtype of the GABA B receptor, but not the 1b subtype, contains a pair of CPs and we present a three-dimensional model of this region.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)00794-7