The extended packaging sequence of MoMLV contains a constitutive mRNA nuclear export function

The present report shows that incorporation of defined sequences from the Moloney murine leukaemia virus (MoMLV) into Rex dependent expression vectors based on the human T-cell leukaemia virus (HTLV-1) allows Rex independent gene expression. Deletion mutagenesis of the MoMLV derived sequences allowe...

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Bibliographic Details
Published in:FEBS letters 1998-09, Vol.434 (3), p.367-371
Main Authors: King, Jason A., Bridger, Joanna M., Gounari, Fotini, Lichter, Peter, Schulz, Thomas F., Schirrmacher, Volker, Khazaie, Khashayarsha
Format: Article
Language:English
Subjects:
Base Sequence
Biological Transport
Cell Line
Cell Nucleus - metabolism
Chloramphenicol O-Acetyltransferase - genetics
DNA Primers
Extended packaging sequence
Gene Products, rex - genetics
HeLa Cells
Human T-cell leukemia virus-1
Human T-lymphotropic virus 1 - genetics
Humans
Leukemia Virus, Murine - genetics
Moloney murine leukemia virus
mRNA nuclear export
Promoter Regions, Genetic
Rex
RNA, Messenger - metabolism
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Summary:The present report shows that incorporation of defined sequences from the Moloney murine leukaemia virus (MoMLV) into Rex dependent expression vectors based on the human T-cell leukaemia virus (HTLV-1) allows Rex independent gene expression. Deletion mutagenesis of the MoMLV derived sequences allowed this function to be localised to a 312 nt length sequence overlapping the MoMLV gag p15/p12 open reading frame. This `extended packaging sequence' has been reported to markedly increase the titre of in vitro packaged retroviral vectors. Using fluorescent in situ hybridisation combined with confocal microscopy we show that the 312 nt element can replace Rex mediated nuclear export and expression of transcripts containing HTLV-1 cis acting repressive elements. Our observations are consistent with the extended packaging sequence of MoMLV exerting a constitutive mRNA nuclear export function.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)00948-X