Induction of outward current by orexin-B in mouse peritoneal macrophages

To define effects of novel feeding regulating peptides, orexins, in immunocompetent cells, ion channel activity in mouse peritoneal macrophages was analyzed by the perforated patch-clamp method. Orexin-B (OX-B) induced an outward current at smaller holding potentials than K + equilibrium potentials....

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Bibliographic Details
Published in:FEBS letters 1998-11, Vol.440 (1), p.51-54
Main Authors: Ichinose, Mitsuyuki, Asai, Masatoshi, Sawada, Masashi, Sasaki, Kazuo, Oomura, Yutaka
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Carrier Proteins - pharmacology
Charybdotoxin - pharmacology
Chlorides - metabolism
Dose-Response Relationship, Drug
Egtazic Acid - pharmacology
Female
Intracellular Signaling Peptides and Proteins
Leptin
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - physiology
Male
Membrane Potentials - drug effects
Mice
Mouse macrophage
Neuropeptides - pharmacology
Orexin
Orexins
Outward current
Patch-clamp
Patch-Clamp Techniques
Potassium - metabolism
Potassium Channel Blockers
Potassium Channels - physiology
Proteins - pharmacology
Quinine - pharmacology
Secretin - pharmacology
Tetraethylammonium - pharmacology
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Summary:To define effects of novel feeding regulating peptides, orexins, in immunocompetent cells, ion channel activity in mouse peritoneal macrophages was analyzed by the perforated patch-clamp method. Orexin-B (OX-B) induced an outward current at smaller holding potentials than K + equilibrium potentials. Reversal potentials of OX-B induced current were dependent on external K + concentrations but not on external Cl − concentration. Orexin-A is less effective than OX-B. Quinine blocked the outward current and tetraethylammonium partially suppressed the current. These results suggest that OX-B can modulate macrophage functions through the activation of Ca 2+-dependent K + channels.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)01432-X