Crocalbin: a new calcium-binding protein that is also a binding protein for crotoxin, a neurotoxic phospholipase A 2

Utilizing Marathon-ready cDNA library and a gene-specific primer corresponding to a partial amino acid sequence determined previously, the complete nucleotide sequence for the cDNA of crocalbin, which binds crotoxin (a phospholipase A 2) and Ca 2+, was obtained by polymerase chain reaction. The open...

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Bibliographic Details
Published in:FEBS letters 1999-02, Vol.445 (2), p.440-444
Main Authors: Hseu, Ming-Jhy, Yen, Chon-Ho, Tzeng, Mu-Chin
Format: Article
Language:English
Subjects:
Calcium-binding protein
Crocalbin
Crotoxin
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Summary:Utilizing Marathon-ready cDNA library and a gene-specific primer corresponding to a partial amino acid sequence determined previously, the complete nucleotide sequence for the cDNA of crocalbin, which binds crotoxin (a phospholipase A 2) and Ca 2+, was obtained by polymerase chain reaction. The open reading frame of the cDNA encodes a novel polypeptide of 315 amino acid residues, including a signal sequence of 19 residues. This protein contains six potential Ca 2+-binding domains, one N-glycosylation site, and a large amount of acidic amino acid residues. The ability to bind Ca 2+ has been ascertained by calcium overlay experiment. Evidenced by sequence similarity in addition, it is concluded that crocalbin is a new member of the reticulocalbin family of calcium-binding proteins.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)00177-5