Loading…

Comparison of the effects of bax-expression in yeast under fermentative and respiratory conditions: investigation of the role of adenine nucleotides carrier and cytochrome c

A new system for bax-expression in yeast has been devised to investigate bax’s effect under fermentative and respiro-fermentative conditions. This has allowed us to show unambiguously that the ability of bax to kill yeast is higher under respiratory conditions than under purely fermentative conditio...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1999-08, Vol.456 (2), p.232-238
Main Authors: Priault, Muriel, Camougrand, Nadine, Chaudhuri, Bhabatosh, Schaeffer, Jacques, Manon, Stéphen
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new system for bax-expression in yeast has been devised to investigate bax’s effect under fermentative and respiro-fermentative conditions. This has allowed us to show unambiguously that the ability of bax to kill yeast is higher under respiratory conditions than under purely fermentative conditions. The extent of killing under respiro-fermentative conditions (non-repressive sugars) is intermediate. It has been proposed that the two proteins adenine nucleotides carrier (ANC) and cytochrome c play a crucial role in bax-induced cell death. We have investigated the effects of deletion of the genes encoding the two proteins on the toxicity induced by bax, using this new system. The absence of ANC did not modify bax-induced lethality in any way. Moreover, the absence of cytochrome c also did not prevent bax-induced death. Only the kinetics of lethality were altered. All these effects are prevented by co-expression of bcl-x L.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)00957-6