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Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae
Four microalgae ( Botryococcus braunii, Chlorella vulgaris, Dunaliella salina, Arthrospira maxima) were object of supercritical CO 2 extraction studies, which were carried out in a flow apparatus at temperatures between 313.1 and 333.1 K and pressures up to 35.0 MPa. The microalga Botryococcus braun...
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Published in: | Inorganica Chimica Acta 2003-12, Vol.356, p.328-334 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | Four microalgae (
Botryococcus braunii,
Chlorella vulgaris,
Dunaliella salina,
Arthrospira maxima) were object of supercritical CO
2 extraction studies, which were carried out in a flow apparatus at temperatures between 313.1 and 333.1 K and pressures up to 35.0 MPa. The microalga
Botryococcus braunii produces extracellular alkadienes. Supercritical extracts obtained at 313.1 K, and pressures of 12.5, 20.0 and 30.0 MPa, were golden and limpid, unlike those obtained with organic solvents. The hydrocarbons of this microalga were selectively extracted and this selectivity increased with pressure. The microalga
Chlorella vulgaris is a carotenoid producer of canthaxanthin and astaxanthin. The extraction yields of lipids and carotenoids were compared for whole, crushed and slightly crushed algae at a pressure of 35.0 MPa and a temperature of 328.1 K. The yield of supercritical extraction of carotenoids was also compared at several conditions of pressure and temperature. It increased with pressure at constant temperature, remained practically constant with temperature, at pressures of 27.5 and 35.0 MPa, and decreased with temperature at 20.0 MPa. The third alga studied was the
Dunaliella salina, which produces β-carotene in high yield. Natural β-carotene (
cis and
trans mixture) from this alga was submitted to supercritical carbon dioxide and it was assessed, at pressures up to 30.0 MPa and temperature of 313.1 K, that both isomers presented higher solubility than the synthetic
trans-β-carotene and that the
cis isomer was much more soluble in supercritical CO
2 than the
trans isomer. Moreover, it was shown that the
cis/
trans ratio, in the supercritical extracts, increased significantly, relatively to the original one in the microalga, when the
Dunaliella was submitted to supercritical CO
2. The cyanobacteria
Arthrospira (
Spirulina)
maxima can produce in large amounts the γ-linolenic acid (GLA), C18:3 ω6. The yield and selectivity of the supercritical extraction, using CO
2 and CO
2 doped with ethanol, of the lipids and GLA were determined and compared with those obtained with organic solvents.
Supercritical CO
2 extraction studies of compounds with pharmaceutical importance from microalgae are presented. These studies involved the
Botryococcus braunii, the
Chlorella vulgaris, the
Dunaliella salina and the cyanobacteria
Arthrospira (Spirulina) maxima, which were used to obtain alkadienes, carotenoids (mainly canthaxanthin and astaxanthin), β-carotene and γ-linol |
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ISSN: | 0020-1693 1873-3255 |
DOI: | 10.1016/S0020-1693(03)00363-3 |