Effect of recombinant(rhu)FXIII on skin wound healing in the streptozotocin-induced diabetes mellitus rat

• Published in: Japanese Journal of Pharmacology 1996, Vol.71 (suppl.1), p.214-214
• Main Authors: Matsuzaki, Tomose, Komine, Isamu, Shinbo, Kazuhiko, Yaguchi, Masafumi, Tsuda, Shuji
• Format: Article
• Language: eng ; jpn
• ISSN: 0021-5198
• DOI: 10.1016/S0021-5198(19)37096-9

In the present study, the effects of human FXHI(rFXIII and plasmaFXIII) on wound healing in the streptozotocin-induced diabetes mellitus rats were investigated It is difficult to examine drug effects on wound healing in normal rodents, because of its superior healing ability to human being. Therefore, she diabetes mellitus rats induced with streptozotocin were used for delayed healing model. The nonenzymatic glycation of proteins induced by hyperglycemia may play a role in the delayed heating process. Four weeks after the treatment with streptozotocin(6Omg/kg iv.), open wound was made in the shaved back skin of each rat under anesthesia. After the surgery, the rats were administered drug every two days. The healing process and period, blood glucose level, and collagen synthesis activity were monitored. rhu FXIII and plasma FXIII improved the healing process of the open wound significantly. Eight days after the surgery, the collagen synthesis activity at the wound skin increased in rhu FXIII and plasma FXIII treated groups. On day 17, blood glucose level in rhu FXIII and plasma FXIII treated groups reduced to 85% of pre treated levels. Although the underlying mechanism of efficacy of FXJII on the wound healing is yet to be clarified, is seems that there is no causal relationship between the healing efficacy and hypoglycemic effect of FXIII, rather the enhancement of collagen synthesis would be a reason for the efficacy. It would be related to improvement of the insufficient extracellular matrix formation caused by glycated fibronectin, through the transglutaminase activity which crosslinks between fibrin, fibronectin, collagen and α ``2 PI.