Comparison of noradrenaline and adenyl purines release from rabbit pulmonary artery and vein

• Published in: Japanese Journal of Pharmacology 1994, Vol.64 (suppl.2), p.326-326
• Main Authors: Takeuchi, Koichi, Akimoto, Hiroko, Shinozuka, Kazumasa, Hattori, Keisuke, Ishii, Reiko, Hashimoto, Takao
• Format: Article
• Language: eng ; jpn
• ISSN: 0021-5198
• DOI: 10.1016/S0021-5198(19)50936-2

We have shown that α_1 -adrenoceptor agonist caused the release of adenine nucleotides and nucleosides from postjunctional sites, probably endothelial cells, in blood vessels (J.Pharmacol.Exp.Ther.,252,1060,1990; Eur.J.Pharmacol., 192,431,1991; Naunyn-Schmiedeberg's Arch. Pharmacol.,348,46,1993). In the present study, we examined the release of endogenous adenyl purines and noradrenaline from electrically stimulated pulmonary artery and vein of rabbit. Adenyl purines and noradrenaline , such as ATP , ADP , AMP and adenos ine , were quantified by HPLC-fluorescence detection and HPLC-electrochemical detection, respectively. The electrical field stimulation (EFS) at 16 Hz increased the overflow of adenyl purines and noradrenaline from the artery and vein. The electrically evoked purines release was completely depressed by prazosin. Molar ratios of the release of purine to noradrenaline in the artery and vein were almost equal, approximately 100 . This observation suggests an important extracellular role for adenyl purines in the physiology of the pulmonary artery and vein of rabbit.