Beneficial Effect of Captopril on Arrhythmia and Cytosolic Acidification in Myocardial Ischemia and Reperfusion in Rat Hearts

• Published in: Japanese Journal of Pharmacology 1993, Vol.61 (suppl.1), p.266-266
• Main Authors: Urabe, Yoshitoshi, Takeshita, Akira, Kanaide, Hideo
• Format: Article
• Language: eng ; jpn
• ISSN: 0021-5198
• DOI: 10.1016/S0021-5198(19)51926-6

Clinical studies have showed that captopril improves prognosis of myocardial infarction. To determine the underlying mechanism for the protective effect, we studied the effect of captopril on cytosolic pH in 15 min regional ischemia followed by reperfusion, using isolated rat hearts perfused with Langendorff procedure(n=31). Ischemia and reperfusion were induced by ligating the left coronary artery at its origin. We recoreded the incidence of arrhythmia (ventricular tachycardia (VT), ventricular fibrillation(Vf)) and myocardial tension, in addition to cytosolic pH. Cytosolic pH was determined using front-surface fluorometer, developed by us, and indicator dye BCECF. Hearts were treated as follows： control ligation only (n=10),captopril (n=11)(150μg/ml,450μg/ml) and angiotensin II (n=10)(10^-7 M). Results were shown in the table (Mean±SEM). These studies show that captopril protects against cytosolic acidosis and arrhythmia in myocardial ischemia and reperfusion.