The role of D1- and D2-dopamine receptor on the function of acetylcholine in the striatum

• Published in: Japanese Journal of Pharmacology 1988, Vol.46 (suppl), p.58-58
• Main Authors: Imamura, Yoshiko, Shibanoki, Shinji, Ishii, Yutaka, Asai, Satoshi, Kubo, Taizo, Ishikawa, Koichi
• Format: Article
• Language: English
• ISSN: 0021-5198; 1347-3506
• DOI: 10.1016/S0021-5198(19)57018-4

Male Wistar rats (250 g) were reserpinized (2 mg/kg) and injected intraperitoneally with the agonist or antagonist of dopamine (DA) receptors to reveal the role of these receptors on the release of acetylcholine (ACh) in the striatum. DA and ACh were simultaneously determined with their metabolites by the high performance liquid chromatography combined with electrochemical detection in the same sample. SCH-23390 (1 mg/kg), a Dl antagonist), increased the concentration of ACH in intact rats. This increasing effect was not observed in reserpinized animals. LY-171555 (1 mg/kg), a D2 agonist, also increased the concentration of ACh in intact animals. The effect of LY was augmented by reserpinization. The synergism was observed between the effects of SCH and LY on ACh level, which was stronger in reserpinized than intact rats. When the reserpinized rats were administered with l-DOPA (100 mg/kg), the concentration of ACh was decreased. This effect was antagonized by pre-treatment of SCH, but not by YM-09151-2 (0.01 mg/kg) , a D2 antagonist. These findings suggest differences in the function of DA receptors on ACh neuronal activity.