Behavioral and biochemical study of experimental cerebral embolization induced by microspheres in unanesthetized rats

• Published in: Japanese Journal of Pharmacology 1986, Vol.40 (suppl), p.251-251
• Main Authors: Sato, Etsuro, Tsukuda, Kenji, Hishiike, Atsuko, Okazaki, Yuko, Taiji, Mutsuo, Irie, Tsunemasa, Katsube, Junki
• Format: Article
• Language: English
• ISSN: 0021-5198; 1347-3506
• DOI: 10.1016/S0021-5198(19)59500-2

We investigated the changes of behavioral and biochemical parameters in microspheres-induced cerebral embolization, which was produced in the unanesthetized rats by injecting 4,000 polystyrene microspheres (40μm in diameter) into the right internal carotid artery. In embolized rats, neurological deficits (paresis of contralateral hind limb, circling behavior and seizure) were observed and the brain water content of ipsilateral hemisphere increased progressively with two peaks at 1hr and 24hr after embolization. Energy metabolic damage with peak at 30 min after embolization occurred in bilateral hemispheres and tended to restore. Severe ischemia were found in ipsilateral striatum, hippocampus and posterior cerebral cortex with carbon black perfusion method. Hippocampal blood flow decreased significantly 30 min after embolization. In these ischemic regions, especially in ipsilateral hippocampus and posterior cortex, significant decrease of ATP content and marked increase of lactate content were observed 1hr after embolization. This cerebral embolization model seems to represent transient and regional ischemic damage.