Partial purification of the antidiuretic hormone receptor from the rabbit kidney

The antidiuretic hormone receptor is located in the plasma membrane of the renal medulla, is a component of the adenyl cyclase system, and physiologically linked to water reabsorption in the collecting ducts. Since the receptor loses its binding activity to the hormone after the membrane is solubili...

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Bibliographic Details
Published in:Japanese journal of pharmacology 1983, Vol.33, p.261-261
Main Authors: Kimura, Koji, Ogura, Naoko, Koseki, Chizuko, Endou, Hitoshi
Format: Article
Language:English
Online Access:Get full text
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Summary:The antidiuretic hormone receptor is located in the plasma membrane of the renal medulla, is a component of the adenyl cyclase system, and physiologically linked to water reabsorption in the collecting ducts. Since the receptor loses its binding activity to the hormone after the membrane is solubilized, the intact membrane was first incubated with 125I-labelled or 3H-labelled arginine vasopressin and then subjected to solubilization with 1.0% Lubrol PX. The receptor-hormone complex was purified by Sepharose CL-6B, DEAE cellulose and SE cellulose chromatography.
ISSN:0021-5198
1347-3506
DOI:10.1016/S0021-5198(19)64852-3