A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence

Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling...

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Bibliographic Details
Published in:The Journal of biological chemistry 1990-11, Vol.265 (31), p.19185-19191
Main Authors: DuBois, R.N., McLane, M.W., Ryder, K, Lau, L.F., Nathans, D
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Base Sequence
Biological and medical sciences
Blotting, Southern
Cell Line
Cloning, Molecular
Colforsin - pharmacology
Escherichia coli - genetics
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Holoproteins
Liver - metabolism
Liver Regeneration
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Nuclear proteins
Nuclear Proteins - biosynthesis
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Proteins
Repetitive Sequences, Nucleic Acid
RNA - genetics
RNA - isolation & purification
RNA, Messenger - drug effects
RNA, Messenger - genetics
Tetradecanoylphorbol Acetate - pharmacology
Transfection
Zinc - metabolism
Zinc Fingers - genetics
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Summary:Growth factors rapidly induce transcription of a set of genes that encode regulatory proteins, many of which have been identified by cDNA cloning. Here we report the analysis of a cDNA corresponding to a gene induced in mouse 3T3 cells by growth factors and a variety of other extracellular signaling agents. The cDNA encodes a proline-, serine-, and glycine-rich nuclear protein designated Nup475 of 319 amino acids that contains two tandemly repeated cysteine- and histidine-containing sequences (CX8CX5CX3H) suggestive of a novel heavy metal-binding domain. Nup475 produced in Escherichia coli binds zinc. Its mRNA is present in a number of mouse tissues and cell lines, being especially abundant in intestine, thymus, and regenerating liver and in a macrophage cell line stimulated by gamma-interferon. We hypothesize that Nup475 is a regulatory protein with a novel zinc finger structure.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)30642-7