Dopaminergic inhibition of DNA synthesis in pituitary tumor cells is associated with phosphotyrosine phosphatase activity

Dopaminergic D2 receptor agonists, such as bromocriptine, are potent anti-proliferative agents in the treatment of human pituitary adenomas. We have reproduced the anti-proliferative effect of dopamine in an established pituitary cell line stably transfected with the rat D2 dopamine receptor cDNA. W...

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Bibliographic Details
Published in:The Journal of biological chemistry 1992-12, Vol.267 (34), p.24169-24172
Main Authors: FLORIO, T, MING-GUI PAN, NEWMAN, B, HERSHBERGER, R. E, CIVELLI, O, STORK, P. J. S
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - metabolism
Animals
Biological and medical sciences
Biological Transport - drug effects
Cell physiology
DNA Replication - drug effects
DNA, Neoplasm - biosynthesis
Dopamine - pharmacology
Fundamental and applied biological sciences. Psychology
Haloperidol - pharmacology
Hormonal regulation
Humans
Kinetics
Molecular and cellular biology
Pertussis Toxin
Pituitary Neoplasms
Protein Tyrosine Phosphatases - metabolism
Rats
Receptors, Dopamine D1 - drug effects
Receptors, Dopamine D1 - genetics
Receptors, Dopamine D1 - physiology
Receptors, Dopamine D2 - drug effects
Receptors, Dopamine D2 - genetics
Receptors, Dopamine D2 - physiology
Somatostatin - pharmacology
Thymidine - metabolism
Transfection
Tritium
Tumor Cells, Cultured
Vanadates - pharmacology
Virulence Factors, Bordetella - pharmacology
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Summary:Dopaminergic D2 receptor agonists, such as bromocriptine, are potent anti-proliferative agents in the treatment of human pituitary adenomas. We have reproduced the anti-proliferative effect of dopamine in an established pituitary cell line stably transfected with the rat D2 dopamine receptor cDNA. We found that dopaminergic inhibition of DNA synthesis parallels the stimulation of a phosphotyrosine phosphatase activity. Both actions are blocked by pertussis toxin and by the phosphotyrosine phosphatase inhibitor, vanadate. We suggest that the anti-proliferative action of dopamine is mediated, at least in part, by the dopaminergic stimulation of a phosphotyrosine phosphatase.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)35744-2