The molecular cloning and characterization of murine ferritin heavy chain, a tumor necrosis factor-inducible gene

Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. We have identified and sequenced a full-length cDNA for murine ferritin heavy chain. The isolated cDNA is 819 nucleotides in length. It includes 546 nucleotides which encode a protein of 182 amino aci...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-09, Vol.263 (25), p.12638-12644
Main Authors: Torti, S V, Kwak, E L, Miller, S C, Miller, L L, Ringold, G M, Myambo, K B, Young, A P, Torti, F M
Format: Article
Language:English
Subjects:
Adipose Tissue - metabolism
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Cell Line
DNA - genetics
DNA - isolation & purification
DNA, Recombinant
Ferritins - biosynthesis
Ferritins - genetics
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation - drug effects
Genetic engineering
Genetic technics
Humans
Methods. Procedures. Technologies
Mice
Molecular and cellular biology
Molecular cloning
Molecular genetics
Molecular Sequence Data
Muscles - metabolism
Protein Biosynthesis
RNA, Messenger - biosynthesis
Sequence Homology, Nucleic Acid
Tumor Necrosis Factor-alpha - pharmacology
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Summary:Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. We have identified and sequenced a full-length cDNA for murine ferritin heavy chain. The isolated cDNA is 819 nucleotides in length. It includes 546 nucleotides which encode a protein of 182 amino acids, a 5′ noncoding sequence of 120 nucleotides, and a 3′-noncoding region of 153 nucleotides. The sequence displays a high degree of homology to human ferritin H, and includes a portion of the iron-responsive element conserved in chick, frog, and human ferritin. Tumor necrosis factor (TNF), a cytokine which mediates elements of the stress response, induces expression of ferritin H mRNA. Both mouse TA1 adipocytes and human muscle cells increase expression of ferritin H mRNA 4-6-fold after 48 h exposure to TNF. This increase occurs both prior and subsequent to differentiation of adipocytes and muscle cells, and is accompanied by an increase in the synthesis of the ferritin H subunit. These findings suggest a novel role for TNF in iron metabolism.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)37801-3