A series of disialogangliosides with binary 2→3 sialosyllactosamine structure, defined by monoclonal antibody NUH2, are oncodevelopmentally regulated antigens

• Published in: The Journal of biological chemistry 1989-11, Vol.264 (31), p.18719-18725
• Main Authors: Nudelman, E D, Mandel, U, Levery, S B, Kaizu, T, Hakomori, S
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - immunology; Analytical, structural and metabolic biochemistry; Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Antigens, Neoplasm - analysis; Antigens, Neoplasm - immunology; Biological and medical sciences; Carbohydrate Conformation; Carbohydrate Sequence; Carbohydrates; Colonic Neoplasms - immunology; Fundamental and applied biological sciences. Psychology; Gangliosides - analysis; Gangliosides - immunology; Humans; Immunization; Immunoglobulin M - immunology; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Molecular Structure; Other biological molecules; Placenta - analysis; Tissue Distribution
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)51527-1

A mouse IgM monoclonal antibody, NUH2, was raised after immunization of mice with the disialoganglioside fraction of human colonic adenocarcinoma. This antibody reacts specifically with disialogangliosides having the Structure 1 shown below. [Display omitted] NUH2 does not react with structures lacking the sialic acid at either the β1→3 or β1→6 side chain (Table I, structure G8), nor with a binary structure having unequal chain lengths (Structure 2 below), nor with a binary type 2 chain structure having a trimannosyl core as found in the side chain of N-linked complex type oligosaccharides (Structure 3 below). [Display omitted] In humans, the disialoganglioside antigens defined by antibody NUH2 are present in low quantity in normal cells (e.g., erythrocytes) and tissues, but are expressed highly in some colonic cancers, placenta, trophoblast, and sperm, and can be regarded as oncodevelopmentally regulated antigens.