Studies on Testosterone Metabolism

Testosterone- 3 H glucuronide- 14 C has been synthesized at the tracer level and injected intravenously into a normal man. The urinary glucuronides and their derivatives were separated by partition chromatography with and without gradient elution. Approximately 20% of the radioactive testosterone gl...

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Bibliographic Details
Published in:The Journal of biological chemistry 1966-12, Vol.241 (24), p.5879-5885
Main Authors: Robel, Paul, Emiliozzi, Roméo, Baulieu, Etienne-Emile
Format: Article
Language:English
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Summary:Testosterone- 3 H glucuronide- 14 C has been synthesized at the tracer level and injected intravenously into a normal man. The urinary glucuronides and their derivatives were separated by partition chromatography with and without gradient elution. Approximately 20% of the radioactive testosterone glucuronide that was injected was found unchanged in the urine and approximately 8% as 5β-androstane-3α,17β-diol- 3 H glucuronide- 14 C having the same 3 H: 14 C ratio. Therefore, a "direct" 5β-metabolism of a steroid glucuronide is demonstrated, indicating that steroid glucuronides can be substrates of oxido reductases. Furthermore, an additional 8% and 3.5% of the injected tritium were found as 5β-androsterone- 3 H glucuronide and sulfate, respectively.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)96352-0