Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent

• Published in: Journal of Chromatography A 2000-11, Vol.897 (1), p.205-214
• Main Authors: Ding, Xiaojing, Mou, Shifen
• Format: Article
• Language: English
• Subjects: Acids - chemistry; Antibiotics; Applied sciences; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Drug Residues - analysis; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; Industrial wastewaters; Milk and cheese industries. Ice creams; Pollution; Polymers - chemistry; Sensitivity and Specificity; Tetracyclines; Tetracyclines - analysis; Wastewaters; Water treatment and pollution
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)00779-2

High-performance ion chromatography (HPIC) is first successfully used to analyze tetracycline antibiotics (TCs) in this work. The TCs are well separated on a solvent compatible polymeric cation-exchange column within 12 min. Isocratic elution with acetonitrile-hydrochloride is very advantageous for routine analysis. HPIC may be seen as a specific variant of the more common high-performance liquid chromatography (HPLC) for water-soluble and polar pharmaceuticals with low hydrophobicity. The detection limits (signal-to-noise ratio=3:1) of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) are 10, 10, 20 and 20 μg l −1, respectively. Samples are prepared by vortex mixing with an ethylenediaminetetraacetic acid disodium salt (Na 2EDTA)–McIlvaine buffer (pH 4.0) solution and the mixture filtrates through a molecular weight cut-off filter. The method has been successfully applied to monitor the OTC removal rate through every reactor in the process of OTC manufacturing wastewater treatment by bio-chemical technology. It is also applicable to determine the TCs residues in milk and milk powder with satisfying results.