High-performance liquid chromatographic method for quantitation of free acids, mono-, di- and triglycerides using an infrared detector

Methyl ester, mono-, di- and triglyceride classes were separated and quantitated by high-performance liquid chromatography. Baseline separations were achieved on an amino-, cyano-bonded Partisil column with a gradient from hexane-chloroform (60:65) to hexane-chloroform-acetonitrile (25:65:35). The e...

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Bibliographic Details
Published in:Journal of Chromatography A 1981-04, Vol.209 (1), p.61-66
Main Authors: Payne-Wahl, Kathleen, Spencer, Gayland F., Plattner, Ronald D., Butterfield, Royden O.
Format: Article
Language:English
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Summary:Methyl ester, mono-, di- and triglyceride classes were separated and quantitated by high-performance liquid chromatography. Baseline separations were achieved on an amino-, cyano-bonded Partisil column with a gradient from hexane-chloroform (60:65) to hexane-chloroform-acetonitrile (25:65:35). The eluate was monitored by an infrared detector set at 5.72 μm, and peak areas were determined by computer integration. Methyl 9,10-dihydroxystearate was used as an internal standard. The total run time and recycling time was 35 min. A precision of 10% or better was obtained for all components, even at the 1% (w/w) level.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)80423-9