Some aspects of the influence of the mobile phase composition in normal-phase liquid-solid chromatography, with special attention to the role of water present in binary organic mixtures

The influence of the mobile phase composition in normal-phase liquid-solid chromatography (LSC) was investigated for binary mixtures of 2,2,4-trimethylpentane withn-butanol and ethyl acetate. The effect of varying water content in these mixtures on the retention was also measured. Carotenoids and si...

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Bibliographic Details
Published in:Journal of Chromatography A 1978-02, Vol.149, p.111-126
Main Authors: Paanakker, J.E., Kraak, J.C., Poppe, H.
Format: Article
Language:English
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Summary:The influence of the mobile phase composition in normal-phase liquid-solid chromatography (LSC) was investigated for binary mixtures of 2,2,4-trimethylpentane withn-butanol and ethyl acetate. The effect of varying water content in these mixtures on the retention was also measured. Carotenoids and simple aromatic solutes with different functional groups were used as solutes and silica Si 60 was used as an adsorbent. The results are discussed in terms of the competition concept for LSC retention, and are related to experimentally observed adsorption isotherms and measured activities of the moderators used. The results show that in all of the mobile phase composition ranges investigated, low water contents influence retention and selectivity. The behaviour of water as a moderator is anomalous in terms of the competition concept, and suggests binary multilayer formation.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)80981-4