Factors influencing the retention of insulins in reversed-phase high-performance liquid chromatographic systems

The effects of the concentrations of methanol, buffer and neutral salt, of pH and temperature, as well as of the type, chain length and concentration of ion-pair reagents added to aqueous phosphoate buffer-methanol eluents upon the retention of bovine and porcine have been investigated in detail. A...

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Bibliographic Details
Published in:Journal of Chromatography A 1982-01, Vol.236 (1), p.51-59
Main Authors: Vigh, Gyula, Varga-Puchony, Zita, Hlavay, József, Papp-Hites, E.
Format: Article
Language:English
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Summary:The effects of the concentrations of methanol, buffer and neutral salt, of pH and temperature, as well as of the type, chain length and concentration of ion-pair reagents added to aqueous phosphoate buffer-methanol eluents upon the retention of bovine and porcine have been investigated in detail. A possible explanation for the retention-influencing effects of both the cationic and anionic ion-pair reagents is proposed.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)82499-1