Gas-liquid chromatography of some alkyl derivatives of 5-fluorouracil

Various procedures for converting 5-fluorouracil into its methyl, butyl and hexyl derivatives are described. Structures were established as the N,N′-dialkyl derivatives using mass spectrometry or combined gas—liquid chromatography—mass spectrometry. The reaction conditions, i.e., the amount of deriv...

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Bibliographic Details
Published in:Journal of Chromatography A 1979-07, Vol.174 (2), p.325-339
Main Authors: de Leenheer, André P., Cosyns-Duyck, M.Cl
Format: Article
Language:English
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Summary:Various procedures for converting 5-fluorouracil into its methyl, butyl and hexyl derivatives are described. Structures were established as the N,N′-dialkyl derivatives using mass spectrometry or combined gas—liquid chromatography—mass spectrometry. The reaction conditions, i.e., the amount of derivatization reagents and reaction time, were optimized. Gas—liquid chromatographic characteristics of the derivatives were investigated on different stationary liquid phases, und 2% or 3% SP-2250, 5% XE-60 and 5% OV-1 were found to be superior. With 5-chlorouracil as the internal standard a linear response for the various derivatives was observed in the microgram range. The applicability of the different dialkyl derivatives in the measurement of 5-fluorouracil in biological materials is discussed.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)86006-9