Elution behaviour of acids in ion-exclusion chromatography using a cation-exchange resin

A large number of strong and weak acids have been subjected to ion-exclusion chromatography by elution with water on a hydrogen-form cation-exchange resin. The column void volume ( V o) and inner volume ( V i ) were determined from a calibration graph obtained by plotting the retention volumes ( V R...

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Bibliographic Details
Published in:Journal of Chromatography A 1979-06, Vol.174 (1), p.153-157
Main Authors: Tanaka, Kazuhiko, Ishizuka, Toshio, Sunahara, Hiroshi
Format: Article
Language:English
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Summary:A large number of strong and weak acids have been subjected to ion-exclusion chromatography by elution with water on a hydrogen-form cation-exchange resin. The column void volume ( V o) and inner volume ( V i ) were determined from a calibration graph obtained by plotting the retention volumes ( V R ) against the first dissociation constants (p KP 1) of the acids. The distribution coefficients ( K d ) of the acids were calculated from the conventional equation for ion-exclusion chromatography: V R = V o + K d V i . As the K d values of most acids are between 0 and 1, it is possible to evaluate by gel chromatography the separation mechanism of acids based on the ion-exclusion effect.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)87045-4