Modified ethylenediamine condensation method and its application in the analysis of catecholamines by ion-exchange chromatography

A modified ethylenediamine condensation method is described in which catecholamines dissolved in a borate buffer are convertedd into fluorescent products by oxidation with hexacyanoferrate(III) in the presence of ethylenediamine under alkaline conditions. The method was applied successfully in the f...

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Bibliographic Details
Published in:Journal of Chromatography A 1978-08, Vol.155 (2), p.415-420
Main Author: Seki, Tokuichiro
Format: Article
Language:English
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Summary:A modified ethylenediamine condensation method is described in which catecholamines dissolved in a borate buffer are convertedd into fluorescent products by oxidation with hexacyanoferrate(III) in the presence of ethylenediamine under alkaline conditions. The method was applied successfully in the fluorimetric determination of catecholamines eluted from a column of weakly acidic ion-exchange resin with a mixed buffer of pH 6.3 containing 0.35 M boric acid and 0.002 M disodium ethylenediaminetetraacetate. An analysis of human urine for catecholamines is described.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)88003-6