Fast polymer liquid chromatography isolation and characterization of plant myrosinase, β-thioglucoside glucohydrolase, isoenzymes

Rapid and high-yielding ion-exchange chromatographic and chromatofocusing methods using fast polymer liquid chromatography ( FPLC) have been developed for plant myrosinase, β-thioglucoside glucohydrolase ( EC 3.2.3.1), isoenzyme studies. The preparative FPLC methods showed a mean recovery of 85% for...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1986-08, Vol.363 (1), p.57-69
Main Authors: Buchwaldt, Lone, Larsen, Lone Melchior, Plöger, Annette, Sørensen, Hilmer
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Rapid and high-yielding ion-exchange chromatographic and chromatofocusing methods using fast polymer liquid chromatography ( FPLC) have been developed for plant myrosinase, β-thioglucoside glucohydrolase ( EC 3.2.3.1), isoenzyme studies. The preparative FPLC methods showed a mean recovery of 85% for six myrosinase preparations from three different plant sources. FPLC chromatofocusing and analytical isoelectric focusing were used to demonstrate the presence of different mixtures of myrosinase isoenzymes in seeds of various crucifers. The stability of the isoenzymes was investigated. The proposed method of plant isoenzyme analysis is briefly discussed in relation to methods previously used for myrosinase isolation and characterization, and studies of glucosinolate catabolism.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)88992-X