Identification of acidic mucopolysaccharides by agarose gel electrophoresis

A micro-method for the identification of most acidic mucopolysaccharides by agarose gel electrophoresis with three different buffer systems is described. In barbital buffer the mucopolysaccharides are fractionated from each other as a function of their net charge, whereas in a diamine buffer the fra...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1977-01, Vol.130, p.299-304
Main Authors: Dietrich, Carl P., McDuffie, Norman M., Sampaio, Lucia O.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A micro-method for the identification of most acidic mucopolysaccharides by agarose gel electrophoresis with three different buffer systems is described. In barbital buffer the mucopolysaccharides are fractionated from each other as a function of their net charge, whereas in a diamine buffer the fractionation is probably achieved according to the degree to which they are bound to the diamine. A combination of barbital and diaminopropane buffers in two-dimensional electrophoresis for the identification of mucopolysaccharides is also described.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)89809-X