Preparative reversed-phase high-performance liquid chromatography of iodinated insulin retaining full biological activity

Insulin monoiodinated in Tyr A14, A19, B16 and B26 can be separated from insulin and diiodoinsulins using reversed-phase high-performance liquid chromatography on LiChrosorb RP-18 columns. Monoiodoinsulins with high and low specific activities were isolated from a number of buffer systems without an...

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Bibliographic Details
Published in:Journal of Chromatography A 1986, Vol.369 (2), p.327-339
Main Authors: Linde, Susanne, Welinder, Benny S., Hansen, Bruno, Sonne, Ole
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Applied sciences
Biological and medical sciences
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Other techniques and industries
Protein hormones. Growth factors. Cytokines
Proteins
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Summary:Insulin monoiodinated in Tyr A14, A19, B16 and B26 can be separated from insulin and diiodoinsulins using reversed-phase high-performance liquid chromatography on LiChrosorb RP-18 columns. Monoiodoinsulins with high and low specific activities were isolated from a number of buffer systems without any reduction in binding affinity and biological activity in isolated rat fat cells. The reason for the previously observed reduction in the binding affinity was probably column bleeding, i.e., chemical degradation of the column support.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)90139-0