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Purification of specific heterologous F(ab) 2 fragments with deae-zeta-prep® cartridges for ion-exchange chromatography
A simple two-step procedure for purifying F(ab) 2 fragments of horse immunoglobulins is described. In the first step, the horse plasma is diluted, made up to 12% (w/v) with ammonium sulphate and digested with pepsin. In the second step, the previously dialyzed solution is chromatographed. Instead of...
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Published in: | Journal of Chromatography A 1988-10, Vol.450 (1), p.133-138 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | A simple two-step procedure for purifying F(ab)
2 fragments of horse immunoglobulins is described. In the first step, the horse plasma is diluted, made up to 12% (w/v) with ammonium sulphate and digested with pepsin. In the second step, the previously dialyzed solution is chromatographed. Instead of a normal ion-exchange resin, a DEAE-cellulose, covalently linked to a synthetic vinyl polymer, was used (DEAE-Zeta-Prep®). With this assembly it is possible to perform chromatography at a high flow-rate without the problems related to the use of large columns. The yield and purity of the final product are satisfactory. This method has been scaled up for industrial application. |
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ISSN: | 0021-9673 |
DOI: | 10.1016/S0021-9673(00)90724-6 |