High-performance gel permeation chromatography with a new type of silica packing material

Highly poroas silica beads in the 10-μm size range with graduated pore sizes were tested as packings for the separation of polystyrene standards. As a result of the high internal porosity of the packings, relatively large capacity factors of the solutes and selectivity factors up to 4.0 were achieve...

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Bibliographic Details
Published in:Journal of Chromatography A 1974-01, Vol.99, p.435-443
Main Authors: Unger, Klaus K., Kern, Rainer, Ninou, Maria C., Krebs, Karl-Friedrich
Format: Article
Language:English
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Summary:Highly poroas silica beads in the 10-μm size range with graduated pore sizes were tested as packings for the separation of polystyrene standards. As a result of the high internal porosity of the packings, relatively large capacity factors of the solutes and selectivity factors up to 4.0 were achieved. On 25-cm columns at u0.1 cm sec the following numbers of theoretical plates were obtained: for ethylbenzene, 4000: for PS 5, 1000: and for PS 411, 400. For all columns, the plot of log M w versus N 12 gave a linear relationship. The selectivity and column efficiency were demonstrated by separating a mixture of PS 411, PS 98, PS 51, PS 19, PS 5 and PS 2 at a flow-rate of 0.1 ml min in 45 min on a 25-cm column.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)90875-6