Chromatographic behaviour of cyclodextrin complexes of NADH and NADP

The retention behaviour of NADH and NADP on an ODS-C 18 reversed-phase column was investigated by using a 0.05 M phosphate buffer (pH 6) containing α-, β- or γ-cyclodextrin (CD) as the mobile phase. Three reversible processes were assumed and the partition coefficients, k 2, of inclusion complexes a...

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Bibliographic Details
Published in:Journal of Chromatography A 1990, Vol.508 (1), p.127-132
Main Authors: Seno, Manabu, Lin, Meiling, Iwamoto, Kazutoshi
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:The retention behaviour of NADH and NADP on an ODS-C 18 reversed-phase column was investigated by using a 0.05 M phosphate buffer (pH 6) containing α-, β- or γ-cyclodextrin (CD) as the mobile phase. Three reversible processes were assumed and the partition coefficients, k 2, of inclusion complexes and their stability constants, K, were calculated. The value of k 2 is clearly dependent on the molecular weight of the CD. It was established from the calculated values of K that NADH and NADP are liable to form more stable inclusion complexes with β-CD than α- and γ-CDs. NADP has a higher stability constant than NADH owing to the formation of hydrogen bonds between the phosphate moieties of NADP and the hydroxyl groups of CDs.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)91245-7