Influence of the structure of macroporous glasses on the separation of polymers by gel permeation chromatography

• Published in: Journal of Chromatography A 1973-01, Vol.77 (1), p.149-159
• Main Authors: Zhdanov, S.P., Belenkii, B.G., Nefedov, P.P., Koromaldi, E.V., Kuznetsova, M.A.
• Format: Article
• Language: English
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)93943-8

The gel permeation chromatographic separation of polystyrenes has been studied on columns of macroporous glass bends differing in the particle size and the pore volume. Macroporous glass beads have been prepared according to the method given in ref. 7 which is described in detail in ref. 2. The possibility of suppressing polymer adsorption on the surface of macroporous glass beads is shown by the addition of small quantities (1–2 %) of the adsorption-active substances ( e.g. tetrahydrofuran) to the eluent. A significant increase in the effectiveness of gel permeation chromatography with increasing pore volume of macroporous glass is observed. The combination of chromatographic columns packed with macroporous glass beads of different sizes which provides the linear calibration relationship in the molecular weight ( M) range of 5 · 10 3–2 · 10 ??? is found. The chromatographic columns of macroporous beads are shown to be no less effective in separating narrow fractions of polystyrene than the styrogel columns of Waters Associates.