Dextran-coated silica packings for high-performance size-exclusion chromatography of proteins

Porous silica beads have excellent mechanical properties for the high-performance liquid chromatography of proteins. However, the importance of non-specific interations between silanols on the silica surface and the proteins requires a modification of these supports before they can be used as statio...

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Bibliographic Details
Published in:Journal of Chromatography A 1988-06, Vol.443, p.55-62
Main Authors: Santarelli, X., Muller, D., Jozefonvicz, J.
Format: Article
Language:English
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Summary:Porous silica beads have excellent mechanical properties for the high-performance liquid chromatography of proteins. However, the importance of non-specific interations between silanols on the silica surface and the proteins requires a modification of these supports before they can be used as stationary phases for size-exclusion chromatography (SEC). Silica beads were coated with dextran bearing a small number of positive charges in order to neutralize the negatively charged silanol groups. Diethylaminoethyl-dextrans (DEAE-dextrans) with a relatively low percentage of dextran units bearing DEAE functions were layered on the silica beads. The influence of several characteristics of these packings on chromatographic performance were studied in order to determine the optimal conditions for the SEC of proteins.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)94782-4