High-performance liquid chromatographic determination of d-/ l-epinephrine enantiomer ratio in lidocaine-epinephrine local anesthetics

A procedure for the determination of the ratio of d∼ to l-epinephrine in lidocaine—epinephrine local anesthetics is described. Epinephrine was isolated via Sep-Pak cartridges and derivatized with 2,3,4,6-tetra-O-acetyl-β- d-glucopyranosyl isothiocyanate; the d- and l-chiral derivatives were separate...

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Bibliographic Details
Published in:Journal of Chromatography A 1985-01, Vol.325 (1), p.249-254
Main Authors: Allgire, James F., Juenge, Eric C., Damo, Cristino P., Sullivan, Gerard M., Kirchhoefer, Ross D.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
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Summary:A procedure for the determination of the ratio of d∼ to l-epinephrine in lidocaine—epinephrine local anesthetics is described. Epinephrine was isolated via Sep-Pak cartridges and derivatized with 2,3,4,6-tetra-O-acetyl-β- d-glucopyranosyl isothiocyanate; the d- and l-chiral derivatives were separated and measured by high-performance liquid chromatography. About 70 samples of various dosage forms and concentrations from four manufacturers were successfully analyzed by the method.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)96025-4