Chromatographic methods for purification of leukocyte interferon

A chromatographic method has been developed for obtaining interferon products of three types: (a) with a specific activity of 10 5 I.U./mg of protein (on the basis of leukocyte interferon); (b) with a specific activity of 10 6 I.U./mg of protein (on the basis of leukocyte interferon); (c) a near-hom...

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Bibliographic Details
Published in:Journal of Chromatography A 1986-09, Vol.364, p.359-368
Main Author: Pasechnik, V.A.
Format: Article
Language:English
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Summary:A chromatographic method has been developed for obtaining interferon products of three types: (a) with a specific activity of 10 5 I.U./mg of protein (on the basis of leukocyte interferon); (b) with a specific activity of 10 6 I.U./mg of protein (on the basis of leukocyte interferon); (c) a near-homogeneous preparation with an activity of 2 · 10 8—4 · 10 8 I.U./mg of protein (for leukocyte and recombinant interferons). In the first two cases, only gel permeation chromatography (GPC) is used, which yields interferon preparations containing the oligomeric forms of interferon and also such biologically active proteins as factor D and interleukin-1. In the last case, GPC and immunoaffinity chromatography may be conveniently supplemented by interferon purification by ion exchange to remove immunoglobulins and the products formed by their hydrolytic or proteolytic decomposition.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)96226-5