Strategy for peak tracking in liquid chromatography on the basis of a multivariate analysis of spectral data

Peak tracking, required for the interpretive optimization of the mobile phase in reversed-phase high-performance liquid chromatography, is performed by means of multiwavelength detection followed by a multivariate analysis of the resulting data. A combination of techniques (target factor analysis an...

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Bibliographic Details
Published in:Journal of Chromatography A 1990-01, Vol.499, p.499-522
Main Authors: Strasters, Joost K., Billiet, Hugo.A.H., de Galan, Leo, Vandeginste, Bernard G.M.
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:Peak tracking, required for the interpretive optimization of the mobile phase in reversed-phase high-performance liquid chromatography, is performed by means of multiwavelength detection followed by a multivariate analysis of the resulting data. A combination of techniques (target factor analysis and iterative target transformation factor analysis) is necessary to circumvent problems caused by a limited resolution or lack of knowledge regarding spectral characteristics and to determine the number of relevant components. A procedure to perform the actual peak tracking, which combines data related to the spectral characteristics and relative peak areas of separated and coeluting components, is presented.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)96996-6