Separation of chlorophylls a and b and related compounds by thin-layer chromatography on cellulose

Chlorophylls a and b, pheophytins a and b, and pheophorbides a and b can be separated from each other and from chlorophyllides a and b on thin layers of cellulose. With precautions, recoveries of chlorophylls and pheophytins exceed 90%, and it is suggested that the method is suitable for quantitativ...

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Bibliographic Details
Published in:Journal of Chromatography A 1965-01, Vol.17, p.322-326
Main Author: Bacon, M.F.
Format: Article
Language:English
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Summary:Chlorophylls a and b, pheophytins a and b, and pheophorbides a and b can be separated from each other and from chlorophyllides a and b on thin layers of cellulose. With precautions, recoveries of chlorophylls and pheophytins exceed 90%, and it is suggested that the method is suitable for quantitative analysis of mixtures of the pigments. Cellulose layers on microscope slides are useful for rapid qualitative work.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)99875-3